These genotypes have high genetic diversity, and they should be considered in prospective breeding programs to develop the mulberry varieties with improved yield and quality, and enhanced tolerance toward adverse environmental stress and diseases.Īljane F, Sdiri N (2016) Morphological, phytochemical and antioxidant characteristics of white ( Morus alba L.), red ( Morus rubra L.) and black ( Morus nigra L.) mulberry fruits grown in arid regions of Tunisia. This was supported by six detected genotypes among mulberry populations based on the UPGMA tree of Nei’s genetic distance, MDS plot, structure analysis and neighbor-net diagram. We determined the optimal value of K (6) by structure analysis and K-means clustering. These results suggested that genetic drift created high genetic differentiation among some populations. Our research outputs were consistent with the high G ST and H T amounts and low rate of N m and H s, which indicating high genetic differentiation of the populations. The results of AMOVA demonstrated the significant genetic interpopulation variations. We detected that the number of different alleles and polymorphic bands together with genetic diversity parameters varied among the populations. Genetic diversity parameters, polymorphism indexes and population structure were detected using GenAlex v. For this purpose, the nuclear genome of ten populations (4–7 individuals per each population) were extracted using the CTAB modified method and amplified using 30 ISSR primers. We investigated genetic diversity and structure of some mulberry Iranian populations to register different genotypes, which could be implemented in further genetic breeding programs. To enhance the species resistance, we need to increase genetic diversity of its varieties using higher genotypes differentiation in breeding programs. Most of the recently developed mulberry varieties have similar narrow genetic base and limited capacity against environmental stress and pathogens. (Mulberry) is an economic and medicinal tree, originated from the Himalaya region and gradually spread all over the world.
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